BioScience Magazine
BioScience Current Issue
The May 2008 Issue of BioScience
- Vol. 58, No. 5 -
Organisms from Molecules to the Environment
Publishing 11 times a year. ISSN 0006-3568.
Cover:These maize callus cells have been manipulated to express green fluorescent protein fused to a bacterial signal peptide. Gold particles, coated with DNA encoding the fluorescent protein and the signal peptide, were fired into the cells using a “gene gun,” or biolistic particle delivery system. Some cells that have been bombarded this way take up and express the DNA-encoded proteins, and thus the technique is widely used in plant biotechnology. The signal peptide localizes the green fluorescent protein to specific regions of interest within the cell; in this image, the red bodies within the cells are the nuclei, stained with propidium iodide. In the article that begins on p. 391, Lorena Moeller and Kan Wang discuss a variety of techniques, including biolistic particle delivery, that are used in research on the production of improved genetically engineered crops such as maize. Photograph: Lorena Moeller and Kan Wang.
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